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Cost-effective development of highly polymorphic microsatellite in Japanese quail facilitated by next-generation
R Tadano1, M Nunome, M Mizutani
1Faculty of Applied Biological Sciences, Gifu University, Gifu, 501-1193, Japan.
Animal Genetics
|October 8, 2014
Summary
New microsatellite markers were developed for Japanese quail, revealing low genetic diversity in experimental lines and high structure among populations. These markers are valuable for future genetic research in Japanese quail.
Area of Science:
- Genomics
- Population Genetics
- Animal Science
Background:
- Next-generation sequencing enables efficient identification of microsatellite loci.
- Microsatellite markers are crucial for assessing genetic diversity and population structure.
Purpose of the Study:
- Develop novel microsatellite markers from Japanese quail genome sequences.
- Characterize genetic diversity and population structure in Japanese quail lines.
Main Methods:
- Developed dinucleotide repeat microsatellite markers.
- Genotyped 385 individuals from 13 Japanese quail lines using autosomal markers.
- Analyzed genetic diversity (alleles, heterozygosity, PIC) and differentiation (FST).
Main Results:
- Identified highly informative microsatellite markers (PIC ≥ 0.50).
- Experimental quail lines exhibited lower genetic diversity compared to the wild-derived line.
- Significant genetic differentiation (FST 0.13-0.83) and clear population clustering were observed.
Conclusions:
- Japanese quail experimental lines are highly structured.
- The developed microsatellite markers are effective for future genetic studies in Japanese quail.
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