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Embryonic stem cell-somatic cell fusion and postfusion enucleation.
1Monash Institute of Medical Research, Monash University, 27-31 Wright Street, Clayton, VIC, 3168, Australia, huseyin.sumer@med.monash.edu.au.
Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2014
Summary
Embryonic stem (ES) cells can reprogram somatic cells through fusion. Researchers developed methods to fuse mouse tetraploid ES cells with somatic cells and isolate reprogrammed somatic cells after removing the ES nucleus.
Area of Science:
- * Stem cell biology
- * Cellular reprogramming
- * Developmental biology
Background:
- * Embryonic stem (ES) cells possess the ability to reprogram somatic cells via cell fusion.
- * Cell hybrids exhibit properties similar to pluripotent cells.
- * Transcriptional changes in heterokaryons can occur without nuclear hybridization.
Purpose of the Study:
- * To investigate the sustained transcriptional changes in somatic cells after removal of the dominant ES nucleus.
- * To establish methods for cell fusion of mouse tetraploid ES cells with somatic cells.
- * To develop techniques for enrichment of heterokaryons and recovery of reprogrammed somatic cells.
Main Methods:
- * Cell fusion between mouse tetraploid ES cells and somatic cells.
- * Enrichment of resulting heterokaryons.
- * Differential removal of the ES cell nucleus.
Main Results:
- * Successful fusion of tetraploid ES cells with somatic cells.
- * Enrichment of heterokaryons achieved.
- * Recovery of somatic cells following selective removal of the ES nucleus.
Conclusions:
- * The described methods enable the study of sustained reprogramming in somatic cells.
- * This approach allows for the isolation of reprogrammed somatic cells.
- * Further research can explore the potential of these reprogrammed cells.
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