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Updated: Apr 23, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
Published on: February 22, 2016
Visualization of epigenetic modifications in preimplantation embryos
Hiroshi Kimura1, Kazuo Yamagata
1Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan, hkimura@bio.titech.ac.jp.
Abstract:
Epigenetic modifications such as DNA methylation and posttranslational histone modifications change drastically during embryonic development. To visualize histone modifications in living embryos, a Fab-based live endogenous modification labeling (FabLEM) technique has been developed. Here we describe the methods required for FabLEM experiments, including Fab preparation from IgG, its conjugation with a fluorescent dye, loading into cultured cells or mouse embryos, and imaging.
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