Related Experiment Video

Updated: Apr 23, 2026

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
12:52

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

16.2K

Step by step manipulation of the CryoCapsule with HPM high pressure freezers

Xavier Heiligenstein1, Ilse Hurbain2, Cédric Delevoye1

  • 1Centre de Recherche, Institut Curie, Paris, France; Structure and Membrane Compartments, CNRS UMR144, Paris, France.

Methods in Cell Biology
|October 8, 2014
PubMed

Keywords:
Correlative light and electron microscopyCryoCapsuleHPM010HPM100High pressure freezingLive-cell imaging

More Related Videos

Single Particle Cryo-Electron Microscopy: From Sample to Structure11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

9.8K
Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy10:01

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Published on: May 1, 2017

15.3K

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.1K