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A simple method for assessing severity of common root rot on barley
Mohammad Imad Eddin Arabi1, Mohammad Jawhar1
1Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria.
The Plant Pathology Journal
|October 8, 2014
Summary
A new method using filter paper effectively assesses barley root rot caused by Cochliobolus sativus. This reliable technique aids breeders in identifying resistant barley cultivars faster.
Area of Science:
- Plant Pathology
- Agronomy
- Molecular Biology
Background:
- Common root rot, caused by Cochliobolus sativus, significantly impacts barley production.
- Developing resistant barley cultivars is crucial for disease management and crop yield.
Purpose of the Study:
- To develop a simple, reliable, and rapid method for assessing common root rot in barley.
- To facilitate the identification of barley genotypes with enhanced resistance to Cochliobolus sativus.
Main Methods:
- Inoculation of sterilized barley subcrown internodes with C. sativus conidial suspension.
- Incubation of inoculated tissues within sandwich filter paper in polyethylene envelopes.
- Comparison of disease assessment results with traditional seedling assays.
Main Results:
- Early detection of disease symptoms within 48 hours post-inoculation.
- Highly significant correlation between the filter paper method and seedling assays across multiple experiments.
- Validation of the filter paper method as a reliable disease assessment tool.
Conclusions:
- The developed sandwich filter paper method offers a rapid and reliable approach for evaluating barley resistance to common root rot.
- This technique is valuable for pre-selection of resistant barley lines under controlled conditions, supporting breeding programs.
