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Published on: September 16, 2019
miR-155 inhibitor reduces the proliferation and migration in osteosarcoma MG-63 cells
Huicheng Lv1, Jun Guo2, Siqin Li3
1Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 404000, P.R. China ; Department of Orthopaedics, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, P.R. China.
Abstract:
As the most common malignant primary bone tumor in childhood, osteosarcoma (OS) maintains a high recurrence, despite the significant improvements in the overall survival rate of high-grade OS patients during the recent decades. Therefore, a novel therapy strategy is required for OS treatment. Recently, various microRNAs (miRNAs or miRs) have been confirmed as deregulated in OS, and the miR-155 dysregulation in OS has been discovered by the microarray analysis. In the present study, the regulation of miR-155 on the OS cell proliferation, migration and invasion on the MG-63 cells was explored in vitro. The miR-155 mimics were found to promote cell proliferation, colony formation, migration and invasion significantly, compared to the control miRNA. An miR-155 inhibitor was also used to evaluate whether miR-155 served as a therapeutic target for OS. The results demonstrated that the miR-155 inhibitor significantly reduced the proliferation, colony formation, migration and invasion of the MG-63 OS cells. Thus, the study confirmed the oncogenic regulation on the OS progression of miR-155, which could serve as a therapeutic target with an miR-155 inhibitor.
Insights
MicroRNA-155 (miR-155) promotes osteosarcoma progression by increasing cell proliferation, migration, and invasion. Inhibiting miR-155 significantly reduced these aggressive behaviors, suggesting it as a potential therapeutic target for osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Osteosarcoma (OS) is the most common pediatric bone cancer with high recurrence rates.
- MicroRNAs (miRNAs) are implicated in OS development and progression.
- Microarray analysis identified dysregulated miR-155 in OS.
Purpose of the Study:
- To investigate the role of miR-155 in regulating osteosarcoma cell proliferation, migration, and invasion.
- To evaluate miR-155 as a potential therapeutic target for osteosarcoma.
Main Methods:
- In vitro experiments using MG-63 osteosarcoma cells.
- Transfection with miR-155 mimics to assess its promoting effects.
- Transfection with miR-155 inhibitor to evaluate its therapeutic potential.
Main Results:
- miR-155 mimics significantly enhanced MG-63 cell proliferation, colony formation, migration, and invasion.
- miR-155 inhibitor significantly suppressed these aggressive cellular behaviors.
- These findings confirm the oncogenic role of miR-155 in osteosarcoma progression.
Conclusions:
- miR-155 acts as an oncomiR in osteosarcoma, driving tumor progression.
- Targeting miR-155 with an inhibitor presents a promising therapeutic strategy for osteosarcoma.
