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Related Experiment Videos

Characterization of vancomycin resistance in Enterococcus faecium and Enterococcus faecalis.

T I Nicas1, C Y Wu, J N Hobbs

  • 1Lilly Research Laboratories, Eli Lilly & Co., Indianapolis, Indiana 46285-0438.

Antimicrobial Agents and Chemotherapy
|July 1, 1989
PubMed
Summary

Vancomycin resistance in Enterococcus faecium is transferable between bacteria. This resistance is inducible and linked to a new membrane protein in the bacteria.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Enterococcus faecium is a significant opportunistic pathogen.
  • Vancomycin is a critical antibiotic for treating resistant infections.
  • Emergence of vancomycin resistance in E. faecium poses a major public health threat.

Purpose of the Study:

  • To investigate the mechanisms of vancomycin resistance in a clinical isolate of Enterococcus faecium.
  • To determine the transferability and inducibility of vancomycin resistance.
  • To identify potential molecular markers associated with resistance.

Main Methods:

  • Conjugation experiments were performed to assess resistance transfer.
  • Inducibility of resistance was evaluated under specific conditions.

Related Experiment Videos

  • Proteomic analysis was employed to identify novel membrane proteins.

    • Main Results:

    • Vancomycin resistance in the clinical isolate E. faecium 180 was confirmed.
    • Resistance was transferable to other enterococcal strains via conjugation.
    • Resistance expression was inducible and correlated with the synthesis of a novel membrane protein.

    Conclusions:

    • Vancomycin resistance in this E. faecium isolate is mediated by a transferable genetic element.
    • Inducible resistance suggests a regulatory mechanism controlled by vancomycin exposure.
    • The newly identified membrane protein may play a role in the resistance phenotype.