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Related Experiment Video

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Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
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Efficient gene delivery and multimodal imaging by lanthanide-based upconversion nanoparticles.

Lin Wang1, Jianhua Liu, Yunlu Dai

  • 1State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, People's Republic of China.

Langmuir : the ACS Journal of Surfaces and Colloids
|October 8, 2014
PubMed
Summary
This summary is machine-generated.

Lanthanide-based upconversion nanoparticles (UCNPs) with polyethylenimine coating offer improved gene delivery and reduced toxicity. These nanoparticles enable efficient gene transfection and trimodality imaging for potential gene therapy applications.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Gene Therapy

Background:

  • Nonviral gene carriers are crucial for gene therapy due to their ease of modification and low immunogenicity.
  • Lanthanide-based upconversion nanoparticles (UCNPs) offer unique optical properties and multimodality imaging capabilities, making them promising for biomedical use.

Purpose of the Study:

  • To develop and evaluate UCNPs coated with polyethylenimine (PEI) as a dual-function platform for efficient gene transfection and trimodality imaging.
  • To assess the biocompatibility and transfection efficiency of the UCNPs-PEI system compared to traditional PEI.
  • To demonstrate the in vitro and in vivo imaging capabilities of the UCNPs for MRI, CT, and upconversion luminescence (UCL).

Main Methods:

  • Synthesized UCNPs and coated them with polyethylenimine (PEI).
  • Performed cytotoxicity assays on UCNPs-PEI compared to PEI polymer.
  • Evaluated gene transfection efficiency using enhanced green fluorescence protein (EGFP) plasmid in Hela cells.
  • Assessed gene silencing efficacy by delivering bcl-2 siRNA into Hela cells.
  • Investigated trimodality imaging (MRI/CT/UCL) capabilities in vitro and in vivo.

Main Results:

  • UCNPs-PEI exhibited significantly lower cytotoxicity than PEI polymer.
  • Achieved higher transfection efficiency of EGFP plasmid DNA into Hela cells using UCNPs-PEI compared to PEI.
  • Demonstrated effective gene silencing of bcl-2 mRNA in Hela cells.
  • Confirmed the utility of UCNPs as contrast agents for MRI, CT, and UCL trimodality imaging both in vitro and in vivo.

Conclusions:

  • UCNPs coated with PEI represent a promising platform for gene therapy, offering enhanced gene transfection efficiency and reduced cytotoxicity.
  • The developed UCNPs possess excellent biocompatibility and versatile trimodality imaging capabilities (MRI/CT/UCL).
  • The facile fabrication and multifunctional properties position UCNPs as a strong candidate for advanced gene therapy and bioimaging applications.