Related Experiment Video
Updated: Apr 22, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Complete Genome Assembly of Enterococcus faecalis 29212, a Laboratory Reference Strain
T D Minogue1, H E Daligault2, K W Davenport2
1Diagnostic Systems Division (DSD), United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Maryland, USA.
Enterococcus faecalis is a Gram-positive bacterium causing diseases like endocarditis. Researchers completed the genome sequencing and annotation of the E. faecalis ATCC 29212 reference strain.
Area of Science:
- Microbiology
- Genomics
- Bacterial Pathogenesis
Background:
- Enterococcus faecalis is a Gram-positive coccus.
- It exists as a commensal organism and a pathogen causing diseases, notably endocarditis.
- The ATCC 29212 strain is a widely utilized reference in laboratory settings.
Purpose of the Study:
- To perform whole-genome sequencing of Enterococcus faecalis ATCC 29212.
- To achieve a "finished" annotated genome assembly for this reference strain.
Main Methods:
- Whole-genome sequencing was performed on E. faecalis ATCC 29212.
- Bioinformatic analysis was used for genome annotation.
- The resulting assembly was characterized as "finished".
Main Results:
- A complete genome sequence for E. faecalis ATCC 29212 was obtained.
- The annotated genome assembly measures approximately 3 Mb.
- The sequencing provides a high-quality reference dataset.
Conclusions:
- The finished genome sequence of E. faecalis ATCC 29212 is now available.
- This resource facilitates further research into E. faecalis biology and pathogenesis.
- It supports studies on antibiotic resistance and virulence factors.
More Related Videos
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
10:18Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
Published on: February 9, 2015
Related Concept Videos
Genome Annotation and Assembly
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Bacterial Phylum Firmicutes
Genomic DNA in Eukaryotes
Bacterial Phylum Verrucomicrobiota
Bacterial Phylum Tenericutes