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Related Concept Videos

Proteomics01:33

Proteomics

7.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
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Proteomic pattern changes associated with obesity-induced asthenozoospermia.

Y Liu1, Y Guo, N Song

  • 1Department of Human Anatomy, Histology and Embryology, Shanghai Key Laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Andrology
|October 9, 2014
PubMed
Summary

Obesity negatively impacts sperm quality and motility by altering key proteins. Declines in ERp57 and ACTRT2 are linked to reduced sperm function in obese men.

Keywords:
Actin-binding-related protein T2 (ACTRT2)asthenozoospermiaendoplasmic reticulum protein 57 (ERp57)obesitysperm protein

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Area of Science:

  • Reproductive Biology
  • Proteomics
  • Men's Health

Background:

  • Obesity is a growing global health concern.
  • Obesity is associated with decreased sperm quality and male fertility issues.

Purpose of the Study:

  • To investigate obesity-associated proteomic changes in human spermatozoa.
  • To identify specific proteins affected by obesity that may impact sperm motility.

Main Methods:

  • Differential proteomic analysis using label-free quantitative LC-MS/MS.
  • Analysis of spermatozoa from obese men with asthenozoospermia and healthy individuals.
  • Validation of protein expression changes using immunofluorescence, Western blot, and flow cytometry.

Main Results:

  • 1975 proteins were identified in the human sperm proteome.
  • 105 proteins were less abundant and 22 were more abundant in obesity-associated asthenozoospermia.
  • Key proteins with reduced expression include endoplasmic reticulum protein 57 (ERp57) and actin-binding-related protein T2 (ACTRT2).

Conclusions:

  • Obesity leads to significant changes in sperm proteome, affecting functions like motility and spermiogenesis.
  • Reduced levels of ERp57 and ACTRT2 in obese men may be critical factors contributing to asthenozoospermia.
  • These findings highlight potential molecular mechanisms linking obesity to male subfecundity.