Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pulmonary Tuberculosis IV

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reviewing hepatitis B virus (HBV)-related mortality in Birmingham (2018-2023): The impact of late diagnosis.

Public health·2026

Same author

Incidence of nosocomial pneumonia and clinical outcomes of patients requiring non-invasive ventilation: A systematic review and meta-analysis.

The Southern African journal of critical care : the official journal of the Critical Care Society·2026

Same author

Response to Letter to Editor: "Estimating the Individualized Effect of Tooth Extraction before Radiotherapy on Osteoradionecrosis Using Causal Machine Learning".

Journal of dental research·2026

Same author

Multi-site validation of an AI-based biomarker test for determining ER, PR, and HER2 status from H&E-stained breast cancer slides.

ESMO real world data and digital oncology·2026

Same author

Estimating the Individualized Effect of Tooth Extraction before Radiotherapy on Osteoradionecrosis Using Causal Machine Learning.

Journal of dental research·2026

Same author

The evolving landscape of leptomeningeal metastases from NSCLC: an international, contemporary, multicenter cohort study.

Annals of oncology : official journal of the European Society for Medical Oncology·2025

Search research articles

Related Experiment Video

Updated: Apr 22, 2026

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis

Published on: July 28, 2023

Is universal sample processing methodology better than conventional techniques for detection of tuberculosis?

V Mittal¹, F Haider, S Singhal

¹Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.

Indian Journal of Medical Microbiology

|October 10, 2014

Summary

The USP method shows higher sensitivity for detecting tuberculosis (TB) in sputum smears compared to conventional methods. However, it is not yet feasible for early TB diagnosis within the Revised National Tuberculosis Control Programme (RNTCP).

More Related Videos

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

Published on: August 11, 2008

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Published on: June 6, 2025

Related Experiment Videos

Last Updated: Apr 22, 2026

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis

Published on: July 28, 2023

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

Published on: August 11, 2008

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Published on: June 6, 2025

Area of Science:

Microbiology
Public Health
Medical Diagnostics

Background:

Accurate identification of infectious tuberculosis cases is vital for global control programs.
Sputum smear microscopy is the primary method for detecting acid-fast bacilli.
Improving the sensitivity of smear microscopy is an urgent necessity.

Purpose of the Study:

To compare the diagnostic accuracy of the USP method with direct smear microscopy and modified Petroff's method.
To evaluate the feasibility of the USP method for early tuberculosis diagnosis.

Main Methods:

197 presumptive tuberculosis patients provided two sputum samples each.
Smears were prepared for direct Ziehl-Neelsen staining.
Two concentration methods, modified Petroff's and USP solution, were used for additional smear preparation and LJ media inoculation.

Main Results:

Out of 93 culture-positive cases, 78.5% were positive by direct smear, 89% by 4% NaOH smear, and 96% by USP smear.
The USP method demonstrated higher sensitivity (96%) compared to modified Petroff's (93%).
The difference in diagnostic accuracy between USP and modified Petroff's was not statistically significant (P>0.01).

Conclusions:

The USP method is more sensitive than conventional smear microscopy techniques for tuberculosis detection.
Despite its increased sensitivity, the USP method is not currently feasible for early tuberculosis diagnosis within the RNTCP.
Further evaluation may be needed to integrate improved diagnostic methods into national control programs.