Related Experiment Video
Updated: Apr 22, 2026
10:54
Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry
Published on: November 21, 2025
746
High-resolution digital profiling of the epigenome
Gabriel E Zentner1, Steven Henikoff2
1Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N, Seattle, Washington 98109, USA.
Nature Reviews. Genetics
|October 10, 2014
Summary
New genome-wide tools offer base-pair resolution for digital epigenomic profiling. These advancements enhance understanding of chromatin
Area of Science:
- Genomics
- Molecular Biology
- Epigenetics
Background:
- Short-read DNA sequencing is widely used for epigenomic analysis.
- Existing methods lack base-pair resolution for detailed epigenomic profiling.
Purpose of the Study:
- To review novel genome-wide tools for high-resolution epigenomic profiling.
- To highlight new insights into gene regulation and chromatin dynamics.
Main Methods:
- Development of methods for footprinting and affinity purification.
- Application of these tools to nucleosomes, RNA polymerases, and transcription factors.
- Utilizing digital epigenomic readouts for in situ analysis.
Main Results:
- Achieved base-pair resolution in epigenomic profiling, a two-order-of-magnitude improvement.
- Enabled detailed analysis of chromatin landscape's role in gene regulation.
- Facilitated direct profiling of turnover kinetics and transcription.
Conclusions:
- Genome-wide tools provide unprecedented resolution for interrogating the epigenome.
- These methods offer new perspectives on gene regulation and chromatin organization.
- Digital epigenomic profiling is advancing our understanding of cellular processes.
