CHD1 and CHD2 are positive regulators of HIV-1 gene expression
Melissa J Rodgers, David J Banks, Kenneth A Bradley
1The Nomis Center for Immunobiology and Microbial Pathogenesis, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA. jyoung@salk.edu.
Background:
Retroviruses encode a very limited number of proteins and therefore must exploit a wide variety of host proteins for completion of their lifecycle.
Methods:
We performed an insertional mutagenesis screen to identify novel cellular regulators of retroviral replication.
Results:
This approach identified the ATP-dependent chromatin remodeler, chromodomain helicase DNA-binding protein 2 (CHD2), as well as the highly related CHD1 protein, as positive regulators of both MLV and HIV-1 replication in rodent and human cells. RNAi knockdown of either CHD2 or the related CHD1 protein, in human cells resulted in a block to infection by HIV-1, specifically at the level of transcription.
Conclusions:
These results demonstrate that CHD1 and CHD2 can act as positive regulators of HIV-1 gene expression.
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