A general approach to spirolactonized Si-rhodamines

Area of Science:

• Organic synthesis
• Chemical biology
• Fluorescent probes

Background:

• Si-rhodamines (SiRBs) are valuable fluorescent dyes.
• Existing synthetic routes may limit structural diversity.
• Targeted labeling of cellular organelles is crucial for biological studies.

Purpose of the Study:

• To develop a general synthetic strategy for spirolactonized Si-rhodamines.
• To create novel SiRB scaffolds with diverse substituents.
• To synthesize and evaluate a near-infrared (NIR) mitochondrial tracker.

Main Methods:

• Developed a general synthetic approach for spirolactonized Si-rhodamines.
• Utilized click chemistry for conjugation.
• Prepared a novel NIR fluorescent probe.

Main Results:

• Demonstrated facile access to various ring-expanded SiRB scaffolds.
• Successfully synthesized a NIR probe targeting mitochondria.
• Observed specific mitochondrial staining in cellular imaging.

Conclusions:

• The new synthetic route offers versatility for creating diverse SiRBs.
• The developed NIR probe enables specific mitochondrial visualization.
• This work expands the toolkit for fluorescent imaging in cell biology.