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New steroidal saponin from Antigonon leptopus Hook. and Arn
Maria Karmella L Apaya1, Christine L Chichioco-Hernandez1
1Institute of Chemistry, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines.
Background:
Antigonon leptopus Hook. and Arn., Polygonaceae (cadena de amor), is a herbal remedy for pain and gout-like symptoms in the Philippines. The methanol extract of A. leptopus have shown strong inhibitory action against xanthine oxidase.
Objective:
To isolate and identify the compound responsible for the xanthine oxidase inhibitory action.
Materials And Methods:
A bioassay-guided isolation scheme using an in vitro assay for the inhibition of xanthine oxidase was employed. The structure was established using spectroscopic analysis and chemical methods.
Results:
The isolated compound was determined to be a noncompetitive inhibitor of xanthine with an IC50 of 1.79 μg/mL.
Conclusion:
The isolated compound may represent a new class of xanthine oxidase inhibitors.
