Esterification of dehydroepiandrosterone by human plasma HDL

Evidence for metabolic esterification of dehydroepiandrosterone (DHEA) in human blood plasma, identification of the active lipoprotein (LP) subclass involved, namely HDL3, as well as positive identification of the long-chain fatty acid esters of DHEA formed as incubation products is presented. The esterification reaction of DHEA and subsequent transfer and transport of DHEA esters in human plasma appears to proceed in a manner similar to that of cholesterol. The experiments presented serve as a model predicting similar metabolic transformations during HDL3 interactions with other steroid hormones that have the delta 5-3 beta-hydroxy steroid ring structure and exhibit nonequilibrium associations with HDL. These observations imply that significant quantities of DHEA, particularly in the conjugated ester form, can enter cells via the membrane receptor-mediated pathways of LP internalization.