Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Postnatal hematologic development in phosphofructokinase-deficient dogs.

J W Harvey1, G R Reddy

  • 1Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610.

Blood
|November 15, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Infection and protection responses of deletion mutants of non-structural proteins of foot-and-mouth disease virus serotype Asia1 in guinea pigs.

Applied microbiology and biotechnology·2021
Same author

Clinical, metabolic, and molecular genetic characterization of hereditary methemoglobinemia caused by cytochrome b<sub>5</sub> reductase deficiency in 30 dogs.

Scientific reports·2020
Same author

Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.

Virus research·2020
Same author

Successful treatment of cardiac dysrhythmia associated with foot and mouth disease in a calf.

Iranian journal of veterinary research·2020
Same author

Serum Iron Analytes in Healthy and Diseased Florida Manatees (Trichechus manatus latirostris).

Journal of comparative pathology·2019
Same author

Expression of bovine interleukin 15 in <i>Pichia pastoris</i> and study on its biological activity: a T-cell activator.

Animal biotechnology·2019
Same journal

Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

Blood·2026
Same journal

Identifying steroid-refractory aGVHD before it happens.

Blood·2026
Same journal

ELISA-negative HIT: antibody recognition and relevance.

Blood·2026
Same journal

EBV and immunodeficiency: the odd couple drawn to the brain.

Blood·2026
Same journal

A bone to pick with ferric carboxymaltose.

Blood·2026
Same journal

A step toward streamlining HIT diagnosis.

Blood·2026
See all related articles

Phosphofructokinase (PFK) deficiency in dogs causes hemolytic anemia due to a lack of the M-type subunit. Newborn dogs have high PFK activity from the L-type subunit, which decreases postnatally.

Area of Science:

  • Canine hematology
  • Enzymology
  • Molecular genetics

Background:

  • Adult dogs with phosphofructokinase (PFK) deficiency exhibit compensated hemolytic anemia.
  • This anemia stems from deficient muscle-type (M-type) phosphofructokinase (PFK) subunit, crucial for erythrocyte PFK activity in adult dogs.

Purpose of the Study:

  • To evaluate the effects of PFK deficiency on hematologic development in dogs.
  • To understand the ontogeny of erythrocyte PFK isozymes in normal and PFK-deficient dogs.

Main Methods:

  • Comparative analysis of routine hematologic parameters in normal and affected dogs from birth.
  • Chromatographic separation of PFK isozymes and enzyme immunoprecipitation studies.
  • Quantification of erythrocyte PFK activity and subunit composition.

Related Experiment Videos

Main Results:

  • Newborn dogs of both groups showed high erythrocyte PFK activity due to predominant liver-type (L-type) subunits.
  • PFK activity and L-type subunit levels decreased dramatically in the first 6-8 weeks of life.
  • The M-type subunit increased postnatally in normal dogs, while affected dogs maintained high reticulocyte counts and low 2,3-diphosphoglycerate (DPG) due to impaired glycolysis.

Conclusions:

  • The shift from L-type to M-type PFK subunits during early life is critical for erythrocyte function in dogs.
  • PFK deficiency impacts hematologic development, leading to persistent anemia and altered erythrocyte metabolism.
  • The study elucidates the developmental changes in erythrocyte PFK and their clinical relevance in canine hemolytic anemia.