Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Calculation of state-to-state differential and integral cross sections for atom-diatom reactions with transition-state wave packets.

The Journal of chemical physics·2014

Same author

[Natural attenuation of tetracycline in the water of Taihu Lake under different environmental conditions].

Huan jing ke xue= Huanjing kexue·2014

Same author

The role of AhR in autoimmune regulation and its potential as a therapeutic target against CD4 T cell mediated inflammatory disorder.

International journal of molecular sciences·2014

Same author

Association between polymorphisms in the flanking region of the TAFI gene and atherosclerotic cerebral infarction in a Chinese population.

Lipids in health and disease·2014

Same author

An Updated Analysis with 85,939 Samples Confirms the Association Between CR1 rs6656401 Polymorphism and Alzheimer's Disease.

Molecular neurobiology·2014

Same author

Immobilized lipase from Candida sp. 99-125 on hydrophobic silicate: characterization and applications.

Applied biochemistry and biotechnology·2014

Same journal

Metal-Organic Framework Monoliths Derived from Emulsion-Templated Foams for Reactive Filtration.

ACS applied materials & interfaces·2026

Same journal

Binary to Quaternary Rare-Earth Phosphates: Compositional Effects on Thermal Properties and CMAS Corrosion Resistance of Environmental Barrier Coatings.

ACS applied materials & interfaces·2026

Same journal

Suture-Free Piezoelectric Band-Aid Membrane for Complex Peripheral Nerve Defects.

ACS applied materials & interfaces·2026

Same journal

Single-Precursor to Dual-Function: A Transformable Metal-Organic Framework Nanoplatform for Photocatalytic H<sub>2</sub> Evolution and CO<sub>2</sub> Reduction.

ACS applied materials & interfaces·2026

Same journal

Surfactant-Templated Synthesis of Mg-Stabilized High-Loading Co Single Atoms in Mesoporous Silica Featuring Robust Co-O Bonds for Efficient Peroxymonosulfate Activation.

ACS applied materials & interfaces·2026

Same journal

Toughening Driven by Interphase Tuning in Bioinspired Nanocomposites: From Structural Engineering to Scalable Fabrication.

ACS applied materials & interfaces·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 22, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

DNA nanostructure-based universal microarray platform for high-efficiency multiplex bioanalysis in biofluids.

Zhenhua Li¹, Bin Zhao, Dongfang Wang

¹Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.

ACS Applied Materials & Interfaces

|October 10, 2014

Summary

This summary is machine-generated.

Researchers developed novel DNA tetrahedron microarrays for high-throughput detection of biomarkers. This platform enables sensitive and specific analysis of microRNA, proteins, and small molecules in clinical samples.

Keywords:

DNA tetrahedron biological fluids biorecognition microarrays multiplex bioanalysis

More Related Videos

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Related Experiment Videos

Last Updated: Apr 22, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Area of Science:

Biotechnology
Nanotechnology
Molecular Diagnostics

Background:

Microarrays are crucial for high-throughput analysis in genomics, proteomics, and clinical assays.
Effective immobilization of biomolecules on solid supports is essential for high-performance microarray fabrication.

Purpose of the Study:

To develop a novel DNA tetrahedron-based microarray platform for versatile bioactive molecule detection.
To evaluate the performance of DNA tetrahedron microarrays for clinical diagnostics.

Main Methods:

Synthesis of DNA tetrahedra with amino groups and DNA extensions via oligonucleotide self-assembly.
Covalent coupling of DNA tetrahedra onto glass substrates to create DNA tetrahedron microarrays.
Application of gap hybridization, sandwich configuration, and aptamer strategies for detecting miRNA, proteins, and small molecules.

Main Results:

DNA tetrahedron microarrays demonstrated enhanced biorecognition capabilities for capturing biomolecules.
Achieved addressable, high-throughput analysis with improved sensitivity and specificity for let-7a miRNA (10 fM), PSA (40 pg/mL), and cocaine (100 nM).
The platform showed excellent performance with clinical serum samples, correlating well with conventional assays.

Conclusions:

A novel approach for fabricating DNA tetrahedron-based microarrays was successfully developed.
The universal platform facilitates the detection of diverse bioactive molecules, showing significant potential for clinical diagnosis.
DNA tetrahedron microarrays offer a promising tool for sensitive, specific, and high-throughput molecular detection.