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Albumin decrease is associated with spontaneous preterm delivery within 48 h in women with threatened preterm labor.

Yujing J Heng1, Lorne Taylor, Brett G Larsen

  • 1Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital , 25 Orde Street, 6-1001, Toronto, ON M5T 3H7, Canada.

Journal of Proteome Research
|October 10, 2014
PubMed
Summary

Threatened preterm labor (TPTL) is linked to changes in maternal leukocyte proteins. Albumin shows a significant decrease, indicating leukocyte activation before preterm birth.

Keywords:
SWATHalbuminiTRAQleukocytespregnancyproteomicsthreatened preterm labour

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Area of Science:

  • Proteomics
  • Maternal-Fetal Medicine
  • Biochemistry

Background:

  • Threatened preterm labor (TPTL) is a major cause of pregnancy-related hospital admissions.
  • Maternal peripheral leukocytes are potential biomarkers for monitoring physiological changes during pregnancy.

Purpose of the Study:

  • To investigate differentially expressed proteins in maternal peripheral leukocytes of women experiencing TPTL.
  • To identify protein biomarkers associated with imminent preterm birth.

Main Methods:

  • Utilized high-throughput mass spectrometry (SWATH and iTRAQ) to analyze leukocyte lysate proteins.
  • Compared protein expression in women with TPTL who delivered preterm within 48 hours versus those who did not.
  • Validated differential protein expression using Enzyme-Linked Immunosorbent Assay (ELISA).

Main Results:

  • SWATH identified 5 differentially expressed proteins, including albumin.
  • iTRAQ identified 14 differentially expressed proteins, including albumin.
  • Albumin was consistently decreased in both methods and validated by ELISA, suggesting it as a biomarker for leukocyte activation.

Conclusions:

  • Decreased albumin levels in maternal leukocytes may indicate leukocyte activation preceding preterm birth.
  • Albumin serves as a potential indicator for threatened preterm labor.
  • Further research is needed to elucidate the role of albumin in TPTL pathophysiology.