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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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A device architecture for three-dimensional, patterned paper immunoassays.

Jeremy E Schonhorn1, Syrena C Fernandes, Anjali Rajaratnam

  • 1Diagnostics For All, 840 Memorial Drive, Cambridge, MA 02139, USA. jschonhorn@dfa.org charles.mace@tufts.edu.

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Summary
This summary is machine-generated.

Researchers developed novel paper-based diagnostic devices for point-of-care testing in resource-limited settings. These inexpensive, easy-to-use immunoassays offer a promising alternative to traditional lab diagnostics for detecting biomarkers like human chorionic gonadotropin (hCG).

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Area of Science:

  • Biomarker detection
  • Point-of-care diagnostics
  • Paper-based assays

Background:

  • Diagnostic assays are crucial for patient health status monitoring, but centralized labs limit point-of-care accessibility.
  • Limited-resource settings require innovative, cost-effective diagnostic tools.
  • Paper is an attractive, inexpensive substrate for developing accessible diagnostic assays.

Purpose of the Study:

  • To describe a novel device architecture for performing immunoassays on patterned paper.
  • To provide design guidelines for paper-based diagnostic devices.
  • To demonstrate the utility of these devices for point-of-care biomarker detection.

Main Methods:

  • Developed a 3D fluidic control system using patterned paper with lateral and vertical wicking.
  • Designed paper-based devices to tune assay duration and performance through patterning.
  • Created a sandwich immunoassay for human chorionic gonadotropin (hCG) in urine.

Main Results:

  • Demonstrated successful detection of human chorionic gonadotropin (hCG) using the paper-based immunoassay.
  • Compared paper-based assay results qualitatively and quantitatively to commercial lateral flow tests.
  • Showcased the potential for broad utility in point-of-care diagnostic applications.

Conclusions:

  • Paper-based diagnostic devices offer a viable, low-cost solution for point-of-care immunoassays.
  • The developed architecture enables precise fluid control for reliable biomarker detection.
  • These devices show significant promise for improving healthcare accessibility in resource-limited settings.