Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

28.7K
Overview
28.7K
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

13.9K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HLA tapasin independence: broader peptide repertoire and HIV control.

Proceedings of the National Academy of Sciences of the United States of America·2020
Same author

Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4<sup>+</sup> T Cells in COVID-19.

Cell·2020
Same author

Comparison of HLA ligand elution data and binding predictions reveals varying prediction performance for the multiple motifs recognized by HLA-DQ2.5.

Immunology·2020
Same author

Key Parameters of Tumor Epitope Immunogenicity Revealed Through a Consortium Approach Improve Neoantigen Prediction.

Cell·2020
Same author

Interferon-γ Release Assay for Accurate Detection of Severe Acute Respiratory Syndrome Coronavirus 2 T-Cell Response.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2020
Same author

Cross-reactive memory T cells and herd immunity to SARS-CoV-2.

Nature reviews. Immunology·2020
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Apr 22, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

14.5K

The immune epitope database (IEDB) 3.0.

Randi Vita1, James A Overton2, Jason A Greenbaum3

  • 1Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, 9420 Athena Circle, CA 92037, USA rvita@liai.org.

Nucleic Acids Research
|October 11, 2014
PubMed
Summary
This summary is machine-generated.

The Immune Epitope Database (IEDB) now offers improved access to immune response data. This update enhances usability for researchers studying adaptive immunity and epitope discovery.

More Related Videos

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

8.9K
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

13.8K

Related Experiment Videos

Last Updated: Apr 22, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

14.5K
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

8.9K
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

13.8K

Area of Science:

  • Immunology
  • Bioinformatics

Background:

  • The Immune Epitope Database (IEDB) is a central resource for immune epitope information.
  • Manual curation of published literature and NIH-funded efforts have built a comprehensive dataset.

Purpose of the Study:

  • To present the IEDB 3.0 release, featuring a redesigned interface.
  • To improve user access to immune epitope data in an intuitive and biologically accurate manner.

Main Methods:

  • Manual curation of >15,000 journal articles and >704,000 experiments.
  • Redesign of the query and reporting interface for IEDB 3.0.
  • Utilizing external ontologies for enhanced data organization.

Main Results:

  • The IEDB now contains a comprehensive dataset of immune epitopes.
  • The IEDB 3.0 release offers an improved user interface for data access.
  • User testing and ontology integration have refined the resource.

Conclusions:

  • The IEDB 3.0 release significantly enhances the accessibility and usability of immune epitope data.
  • The updated resource supports ongoing research in adaptive immunity and epitope discovery.
  • Continued curation ensures the IEDB remains a vital, up-to-date information source.