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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

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Related Experiment Video

Updated: Apr 22, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Gateway®-compatible plant transformation vectors.

Mark A Smedley¹, Wendy A Harwood

¹Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK, mark.smedley@jic.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|October 11, 2014

Summary

This summary is machine-generated.

This study presents a fast and efficient cloning protocol for creating over-expression binary vectors. These vectors are crucial for Agrobacterium-mediated plant transformation, aiding functional genomics and crop improvement.

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Last Updated: Apr 22, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

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Published on: January 3, 2014

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

Area of Science:

Plant Biotechnology
Functional Genomics
Molecular Biology

Background:

Plant functional genomics and crop improvement rely on transgene expression.
Successful transgene expression requires a plasmid vector and an efficient delivery system.
Agrobacterium-mediated transformation using binary plasmid vectors is the standard method for plant gene delivery.

Purpose of the Study:

To provide an overview of Gateway®-compatible binary vectors for Agrobacterium-mediated plant transformation.
To describe a rapid, efficient, and robust cloning protocol for generating over-expression binary vectors.

Main Methods:

Utilized Gateway® recombinational cloning technology.
Developed a cloning protocol for constructing over-expression binary vectors.
Applied Agrobacterium-mediated transformation systems for transgene delivery.

Main Results:

Successfully established a fast, efficient, and robust cloning protocol.
Produced an over-expression binary vector suitable for Agrobacterium-mediated transformation.
Demonstrated the utility of Gateway® cloning for constructing plant transformation vectors.

Conclusions:

The described Gateway® cloning protocol streamlines the production of binary vectors for plant transformation.
This method supports high-throughput functional genomics and crop improvement initiatives.
Efficient cloning techniques are essential for advancing plant biotechnology.