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Related Experiment Video

Updated: Apr 22, 2026

Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
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Setaria viridis.

Joyce Van Eck1, Kerry Swartwood

  • 1Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, NY, 14853, USA, jv27@cornell.edu.

Methods in Molecular Biology (Clifton, N.J.)
|October 11, 2014
PubMed
Summary

Developing a model plant for C4 grasses like Setaria viridis requires efficient gene transfer. This study details Agrobacterium tumefaciens-mediated transformation, enabling functional genomics research in this important grass species.

Area of Science:

  • Plant biology
  • Genomics
  • Biotechnology

Background:

  • Plant model systems offer advantages like rapid generation times and small stature for genetic studies.
  • C4 photosynthetic grasses present challenges for research, including long generation times and large space requirements.
  • Setaria viridis is a potential model species for C4 grasses but requires established gene transfer methods.

Purpose of the Study:

  • To establish a reliable gene transfer methodology for Setaria viridis.
  • To facilitate functional genomics studies in C4 grasses using Setaria viridis as a model.
  • To overcome practical limitations hindering research on C4 photosynthetic grasses.

Main Methods:

  • Agrobacterium tumefaciens-mediated transformation of seed-derived callus in Setaria viridis.

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  • Utilization of vectors containing the hygromycin phosphotransferase gene for selection.
  • Regeneration and growth of transgenic plants from transformed callus.

    • Main Results:

    • Successful Agrobacterium tumefaciens-mediated transformation of Setaria viridis.
    • Straightforward transition of transgenic lines from callus to soil-grown plants.
    • Rapid flowering (1-3 weeks) and seed harvest (approx. 5 weeks) of transgenic Setaria viridis.

    Conclusions:

    • The described method enables efficient gene transfer in Setaria viridis, supporting its use as a model for C4 grass functional genomics.
    • This advancement addresses key challenges in studying C4 grasses, paving the way for accelerated research.
    • Setaria viridis is now a viable model for genetic and genomic studies due to the established transformation protocol.