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Related Experiment Video

Updated: Apr 22, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
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Sorghum (Sorghum bicolor).

Xiaomei Guo1, Zhengxiang Ge, Shirley J Sato

  • 1Department of Agronomy and Horticulture, Center for Plant Science Innovation, George W. Beadle Center, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE, 68588-0665, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 11, 2014
PubMed
Summary

Agrobacterium-mediated transformation enables introducing new traits into sorghum (Sorghum bicolor L. Moench) using immature embryos. This method yields primary transgenic events within 4-6 months, with traits inherited predictably.

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Area of Science:

  • Plant Biotechnology
  • Genetics and Molecular Biology
  • Agricultural Science

Background:

  • Sorghum (Sorghum bicolor L. Moench) genetic improvement is crucial for global food security.
  • Agrobacterium-mediated transformation is a key tool for introducing novel traits into crops.
  • Efficient transformation protocols are essential for advancing sorghum breeding programs.

Purpose of the Study:

  • To outline and optimize the protocol for Agrobacterium-mediated transformation of sorghum.
  • To detail the requirements for maintaining stock plants for consistent explant supply.
  • To establish a reliable method for generating primary transgenic sorghum events.

Main Methods:

  • Utilizing immature embryo explants for Agrobacterium transformation.
  • Maintaining high-quality stock plants under controlled greenhouse conditions.
  • Implementing strategies for pollen dispersal monitoring and head bagging to ensure genetic integrity.

Main Results:

  • The transformation protocol successfully generates transgenic sorghum.
  • Primary transgenic events are established within a 4 to 6-month timeframe.
  • Transgenic alleles are typically inherited as a single functional locus, simplifying downstream breeding.

Conclusions:

  • Agrobacterium-mediated transformation of immature sorghum embryos is an effective strategy for crop improvement.
  • The described protocol ensures a consistent supply of explants and reliable generation of transgenic events.
  • The inheritance pattern of transgenic alleles facilitates efficient integration into breeding lines.