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Related Experiment Video

Updated: Apr 22, 2026

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

Published on: September 7, 2022

Tomato (Solanum lycopersicum).

Dora Garcia¹, Javier Narváez-Vásquez, Martha L Orozco-Cárdenas

¹Plant Transformation Research Center, University of California, Riverside, CA, 92507-0124, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 11, 2014

Summary

This study details Agrobacterium-mediated transformation for genetically modifying tomato plants. It provides methods for gene integration analysis and expression verification in the Micro-Tom cultivar.

Area of Science:

Plant Biotechnology
Molecular Biology
Genetics

Background:

Agrobacterium-mediated transformation is a key technique for genetic modification in Solanaceae species.
Tomato (Solanum lycopersicum) is an important model and crop species for genetic studies.

Purpose of the Study:

To describe a protocol for the genetic transformation of tomato cultivar Micro-Tom.
To detail methods for assessing transgene integration and expression.

Main Methods:

Agrobacterium-mediated transformation using cotyledon explants from Micro-Tom tomato cultivar.
Duplex quantitative PCR (qPCR) TaqMan assay for determining gene-copy number.
Histochemical analysis for assessing GUS (beta-glucuronidase) reporter gene expression.

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Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

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Last Updated: Apr 22, 2026

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

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Published on: September 7, 2022

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

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Published on: March 16, 2010

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Main Results:

Successful genetic transformation of tomato Micro-Tom cultivar was achieved using cotyledon explants.
The protocol includes reliable methods for quantifying transgene copy number.
Histochemical analysis confirmed the expression of the introduced gene.

Conclusions:

The described protocol provides a robust method for the genetic transformation of tomato Micro-Tom.
Accurate assessment of gene-copy number and expression is crucial for transgenic plant development.
This methodology can be applied to other Solanaceae species.