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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Apr 22, 2026

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

An Improved Method for Soil DNA Extraction to Study the Microbial Assortment within Rhizospheric Region.

Faria Fatima¹, Neelam Pathak¹, Smita Rastogi Verma²

¹Department of Biosciences, Integral University, Lucknow 226026, India.

Molecular Biology International

|October 11, 2014

Summary

Optimizing soil DNA extraction is crucial for understanding microbial diversity. A new method using phosphate buffered saline (PBS) and mannitol improves DNA yield and purity, enhancing microbial community analysis.

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Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

Area of Science:

Microbiology
Molecular Biology
Soil Science

Background:

Soil microbial communities are vital for ecosystem functions.
Accurate identification relies on effective soil DNA extraction.
Common challenges include incomplete cell lysis and humic substance contamination.

Purpose of the Study:

To evaluate and compare five soil DNA extraction protocols.
To identify an optimized method for high-purity and high-yield DNA recovery.
To assess the impact of DNA extraction on microbial community profiling.

Main Methods:

Investigated five distinct DNA extraction protocols for rhizospheric soil.
Incorporated 120 mM phosphate buffered saline (PBS) for washing.
Utilized mannitol in the lysis buffer for enhanced cell disruption.
Analyzed DNA purity and yield.
Assessed microbial community composition using random amplification of polymorphic DNA (RAPD).

Main Results:

The optimized protocol, using PBS and mannitol, significantly reduced processing time and equipment needs.
Improved DNA purity and yield were achieved compared to other methods.
Random amplification of polymorphic DNA (RAPD) patterns demonstrated method-dependent variations in microbial abundance and composition.
Effective DNA recovery facilitated metagenomic DNA amplification.

Conclusions:

The inclusion of PBS and mannitol in soil DNA extraction protocols enhances DNA yield and purity.
Optimized DNA extraction methods are essential for accurate characterization of soil microbial communities.
This improved method allows for better exploitation of the genetic potential within soil samples.