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Updated: Apr 22, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
A review of methods to synthesise 4'-substituted nucleosides
Mark Betson1, Nigel Allanson, Philip Wainwright
1Peakdale Molecular, Sheffield Road, Chapel-en-le-Frith, Derbyshire SK23 0PG, UK. mark.betson@peakdale.co.uk.
Abstract:
Modified nucleosides have received a great deal of attention from the scientific community, either for use as therapeutic agents, diagnostic tools, or as molecular probes. Perhaps the most difficult position of a nucleoside to modify is the 4'-position. Chemists have developed innovative methods to achieve this in a stereoselective manner to allow incorporation of a variety of functional groups. This review provides a summary of the most commonly used or recently published methods for ribose, deoxy-ribose, 4'-thioribose, and carbocyclics.
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