Related Experiment Video
Updated: Apr 22, 2026

Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia
Published on: June 28, 2018
Stenotrophomonas maltophilia Pseudo-outbreak at a University Hospital Bronchoscopy Unit in Turkey
G Ece1, B Erac2, M H Limoncu2
1Department of Medical Microbiology, Izmir University School of Medicine,Yeni Girne Boulevard, 1825th Street, 12 Karsiyaka, Izmir, Turkey. gulfem.ece@gmail.com.
Objective:
Stenotrophomonas maltophilia is an opportunistic pathogen found predominantly in the environment and hospital setting. Invasive procedures and treatment methods, instruments used for diagnosis and irrational antibiotic use play major roles in the spread of this pathogen. The study aimed to evaluate consecutive S maltophilia isolation from bronchoalveolar lavage samples during bronchoscopy procedure during a week.
Methods:
Four patients consecutively had S maltophilia isolated during bronchoscopy between September 8 and 15, 2012. The identification of the isolates and their antibiotic susceptibility were studied by automated Vitek version 2.0 (Biomerieux, France) system. The clonal relationship between the isolates was studied by enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR).
Results:
Four consecutive S maltophilia isolates had identical band patterns and showed clonal relatedness.
Conclusion:
Bronchoscopy is a common invasive procedure that is utilized in chest diseases departments and intensive care units (ICUs). Contamination may take place due to inappropriate use and cause spread of infectious pathogens. In the current study, we detected consecutive S maltophilia strains with identical band patterns isolated within a week. After appropriate disinfection and cleaning procedures, no further isolation was detected.
Insights
This study found identical Stenotrophomonas maltophilia strains during bronchoscopy, suggesting potential equipment contamination. Implementing strict cleaning protocols halted further S. maltophilia isolation, highlighting the importance of disinfection in preventing pathogen spread.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Clinical Diagnostics
Background:
- Stenotrophomonas maltophilia is an opportunistic pathogen frequently found in healthcare settings.
- Its spread is often linked to invasive procedures, diagnostic tools, and antibiotic misuse.
- Bronchoscopy is a common procedure where pathogen transmission can occur.
Purpose of the Study:
- To investigate consecutive isolations of S. maltophilia from bronchoalveolar lavage samples during bronchoscopy.
- To determine the clonal relationship between S. maltophilia isolates obtained within a short timeframe.
Main Methods:
- Collected bronchoalveolar lavage samples from four patients with S. maltophilia during bronchoscopy.
- Identified isolates and assessed antibiotic susceptibility using the Vitek 2.0 system.
- Analyzed clonal relatedness via enterobacterial repetitive intergenic consensus (ERIC) PCR.
Main Results:
- Four consecutive S. maltophilia isolates exhibited identical band patterns on ERIC PCR, indicating clonal relatedness.
- The findings suggest a potential common source of infection, possibly related to the bronchoscopy procedure.
Conclusions:
- Consecutive S. maltophilia strains with identical genetic profiles were identified within a week during bronchoscopy.
- Adherence to appropriate disinfection and cleaning protocols effectively prevented further isolation of the pathogen.
- This highlights the critical role of instrument decontamination in preventing healthcare-associated infections.
More Related Videos
03:22Author Spotlight: Advancing Pathogen Detection and Disease Assessment in Real-Time Using M-ROSE
Published on: March 1, 2024
10:26P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
Published on: June 15, 2020
Related Concept Videos
Atypical Pneumonia
Endoscopic Studies I: Bronchoscopy and Thoracoscopy
Bronchoscopy
Description
Bronchoscopy is a procedure that involves direct visualization of the larynx, trachea, and bronchi for diagnostic and therapeutic purposes. A flexible fiber optic or rigid bronchoscope is used to carry out the procedure. The fiber-optic bronchoscope is more frequently used due...
Microbiota of the Respiratory Tract
Pulmonary Tuberculosis IV
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Pneumonia IV: Management
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include: