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Related Experiment Videos

Developmental patterns for pancreatic opioids in the rat.

A M Powell1, N R Voyles, S D Wilkins

  • 1Diabetes Research Laboratory, Veterans Administration Medical Center, Washington, DC 20422.

Pancreas
|January 1, 1989
PubMed
Summary

Rat pancreatic opioid peptides and islet hormones surge during the first postnatal week, except for enkephalin precursors which decrease after fetal development. This suggests a role in endocrine pancreas maturation.

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Area of Science:

  • Endocrinology
  • Developmental Biology
  • Neuroendocrinology

Background:

  • The endocrine pancreas develops throughout gestation and early life.
  • Opioid peptides and hormones play crucial roles in physiological regulation.
  • Understanding their developmental trajectories is key to comprehending pancreatic function.

Purpose of the Study:

  • To investigate the developmental patterns of opioid peptides and islet hormones in rat pancreas.
  • To correlate changes in these substances with pancreatic development from fetal stages to adulthood.

Main Methods:

  • Radioimmunoassays were used to measure insulin, glucagon, somatostatin, beta-endorphin, Met- and Leu-enkephalins, and their precursors.
  • Tissue samples were collected from gestational day 20 through 7 weeks postnatal.

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  • Opioids were purified using reversed-phase C-18 cartridges, and precursors were digested prior to measurement.

    • Main Results:

    • Opioid peptides and hormones (insulin, glucagon, somatostatin) exhibited a significant surge in pancreatic concentration during the first postnatal week.
    • Enkephalin precursor concentrations were highest in the fetus and decreased by over 50% by postnatal day 7.
    • These developmental patterns resemble previously observed surges in thyroid-releasing hormone (TRH) in neonatal rat islets.

    Conclusions:

    • The study identifies distinct developmental patterns for opioid peptides and islet hormones in the rat pancreas.
    • The observed postnatal surges suggest a critical role for these substances in the maturation of the endocrine pancreas.
    • The decrease in enkephalin precursors may indicate the maturation of specific enzymatic pathways within the pancreatic islets.