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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Apr 22, 2026

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

Microfluidic isolation of nucleic acids.

Sarah J Reinholt¹, Antje J Baeumner

¹Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853 (USA).

Angewandte Chemie (International Ed. in English)

|October 14, 2014

Summary

This summary is machine-generated.

Efficient nucleic acid (NA) isolation is key for microfluidic point-of-care systems. This review explores various microfluidic techniques for NA purification, aiming for improved efficiency.

Keywords:

analytical methods microfluidics nucleic acid isolation μTAS

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Last Updated: Apr 22, 2026

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

Microfluidic Applications for Disposable Diagnostics

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

Nucleic acid (NA) detection in micro total analysis systems (μTASs) is crucial for point-of-care diagnostics.
Efficient NA isolation is a critical bottleneck for the effectiveness of these systems.
Microfluidics offers advantages like reduced sample/reagent use, increased speed, and automation for NA isolation.

Purpose of the Study:

To review and analyze various microfluidic techniques for nucleic acid isolation.
To discuss considerations for selecting appropriate microfluidic NA isolation methods.
To highlight advantages, disadvantages, and applications of different miniaturized NA purification strategies.

Main Methods:

Silica-based surfaces
Functionalized paramagnetic beads
Oligonucleotide-modified polymer surfaces
pH-dependent charged surfaces
Al2O3 membranes
Liquid-phase isolation

Main Results:

Microfluidic devices enable novel NA isolation techniques not feasible on a macro scale.
Various methods offer different trade-offs in efficiency, cost, and complexity.
The review consolidates information on diverse microfluidic NA isolation approaches.

Conclusions:

Microfluidic platforms are highly suitable for miniaturized NA purification.
Understanding the strengths and weaknesses of each technique is vital for method selection.
Further development in NA isolation chemistry is needed to achieve higher efficiencies in microfluidic systems.