Related Experiment Video
Updated: Apr 22, 2026
07:42
A Data-Driven Approach to Quantifying Immune States in Sepsis
Published on: February 7, 2025
595
[Proteomics and sepsis: new perspectives for diagnosis]
Afonso J C Soares1, Marise F Santos1, Janete Chung1
1IQ, UFRJ.
Revista Brasileira De Terapia Intensiva
|October 14, 2014
Summary
Proteomics analysis revealed distinct serum protein profiles in sepsis patients compared to healthy individuals. This study identifies potential biomarkers for sepsis diagnosis and therapeutic targets.
Area of Science:
- Biochemistry
- Proteomics
- Clinical Chemistry
Context:
- Sepsis diagnosis and treatment remain challenging, necessitating novel approaches.
- Differential protein expression in sepsis serum is not fully understood.
Purpose:
- To investigate differential protein expression in the serum of sepsis patients versus healthy controls using proteomics.
- To identify potential protein biomarkers for sepsis.
Summary:
- Serum samples from 30 sepsis patients and 30 healthy controls were analyzed using two-dimensional gel electrophoresis (2D-SDS-PAGE) and mass spectrometry (MALDI TOF-TOF).
- Proteomic analysis identified several differentially expressed proteins, including acute-phase proteins (e.g., Haptoglobin, Serum amyloid A-1) and lipoproteins (e.g., Apolipoprotein A-IV).
Impact:
- This study provides a proteomic characterization of serum protein alterations in sepsis.
- Findings suggest the potential for developing a diagnostic protein panel and identifying new therapeutic targets for sepsis.
Related Concept Videos
01:33Proteomics
7.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.4K
01:19MALDI-TOF Mass Spectrometry
5.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.7K