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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Related Experiment Video

Updated: Apr 22, 2026

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

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The association between circulating microRNA levels and coronary endothelial function.

R Jay Widmer1, Woo-Young Chung1, Joerg Herrmann1

  • 1Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic and College of Medicine, Rochester, Minnesota, United States of America.

Plos One
|October 14, 2014
PubMed
Summary

Patients with early coronary endothelial dysfunction show elevated transcoronary gradients of specific microRNAs (miRs), suggesting their involvement in early atherosclerosis. Further research is needed to understand the mechanistic role of these miRs.

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Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Genetics

Background:

  • Human microRNAs (miRs) regulate gene expression and are linked to various diseases.
  • The role of miRs in the early stages of coronary atherosclerosis and coronary endothelial dysfunction (CED) remains largely unknown.
  • Investigating transcoronary miR gradients may offer insights into early cardiovascular disease mechanisms.

Purpose of the Study:

  • To determine if patients with early atherosclerosis and CED exhibit altered transcoronary microRNA gradients.
  • To test the hypothesis that microvascular CED is associated with specific changes in circulating miRs across the coronary vasculature.

Main Methods:

  • Coronary angiography and endothelial function testing were performed on patients.
  • Patients were categorized into groups with normal and abnormal microvascular endothelial function based on acetylcholine infusion response.
  • Blood samples were collected simultaneously from the aorta and coronary sinus for microRNA analysis.

Main Results:

  • Patients with microvascular CED showed significantly elevated transcoronary gradients for miR-92a and miR-133.
  • A significant inverse correlation was observed between the percent change in coronary blood flow (CBF) and the transcoronary gradient of miR-133.
  • No significant differences in baseline characteristics were noted between the groups.

Conclusions:

  • Selected microRNAs, specifically miR-92a and miR-133, demonstrate elevated transcoronary gradients in patients with microvascular coronary endothelial dysfunction.
  • These findings highlight a potential role for specific miRs in the early pathogenesis of coronary atherosclerosis.
  • Further investigation into the mechanistic involvement of miRs in human coronary atherosclerosis is warranted.