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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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Practical considerations in comprehensive two-dimensional liquid chromatography systems (LCxLC) with reversed-phases
Duxin Li1, Cornelia Jakob, Oliver Schmitz
1Applied Analytical Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Analytical and Bioanalytical Chemistry
|October 15, 2014
Summary
Online comprehensive two-dimensional liquid chromatography (LCxLC) offers high peak capacity for complex samples. This review focuses on reversed-phase (RPxRP) systems, detailing practical development and application considerations for enhanced separation performance.
Area of Science:
- Analytical Chemistry
- Chromatography Science
Background:
- One-dimensional liquid chromatography (LC) has limitations in separating complex mixtures.
- Online comprehensive two-dimensional liquid chromatography (LCxLC) significantly enhances peak capacity.
- Reversed-phase (RPxRP) column coupling is a powerful LCxLC configuration for complex sample analysis.
Purpose of the Study:
- To review practical considerations for developing and applying RPxRP LCxLC systems.
- To highlight strategies for optimizing separation performance and speed in RPxRP chromatography.
- To address challenges in achieving broad fraction coverage in RPxRP separations.
Main Methods:
- Systematic investigation of column stationary phase chemistry.
- Evaluation of different column combinations and mobile phase systems.
- Analysis of interface design and gradient elution strategies for LCxLC.
- Exploration of methods to improve fraction coverage in RPxRP systems.
Main Results:
- RPxRP coupling provides a high peak capacity product rate.
- Optimization of stationary phases, mobile phases, and interfaces is crucial for effective RPxRP.
- Fast analysis is achievable in LCxLC systems.
- Correlated separation mechanisms in RPxRP limit fraction coverage.
Conclusions:
- RPxRP LCxLC is a versatile and promising technique for complex sample separation.
- Addressing fraction coverage limitations is key to maximizing RPxRP utility.
- This review provides practical guidance for RPxRP system development and application.
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