Related Experiment Video
Updated: Apr 22, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Comparison of Raman spectroscopy and two molecular diagnostic methods for Burkholderia cepacia complex species
C C Orelio1, S H W Beiboer, M C Morsink
1Research Department of Innovative Molecular Diagnostics, University of Applied Sciences Leiden, J. H. Oortweg 21, 2333 CD Leiden, The Netherlands.
Objectives:
Burkholderia cepacia complex (Bcc) and Pseudomonas aeruginosa strains, colonize the respiratory tract of cyctic fibrosis patients. These strains are phenotypically difficult to discriminate, but differ greatly in their pathogenic potential and species identification is relevant. Here, three methods were compared for their diagnostic capacity.
Methods:
A Bcc collection was analyzed with Raman spectroscopy, AFLP and rep-PCR analysis.
Results:
Raman spectroscopy of 40 strains revealed high similarity. Rep-PCR and AFLP of respectively 96 and 112 strains revealed that Bcc strains could be distinguished from Pseudomonas strains. Both molecular methods allowed the identification of most Bcc species according to previous phenotypic and molecular characterization.
Conclusion:
Both AFLP and rep-PCR method data correspond with the previously reported species identification. However, Raman spectroscopy does not discriminate among P. aeruginosa and Bcc species and is therefore not useful as a diagnostic tool.
More Related Videos
08:31Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
11:09Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA
Published on: January 2, 2015
Related Concept Videos
Methods of Classification and Identification
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
MALDI-TOF Mass Spectrometry