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Updated: Apr 22, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Investigation of membrane protein-protein interactions using correlative FRET-PLA
Daniel Ivanusic1, Magdalena Eschricht2, Joachim Denner2
1Robert Koch Institute, Berlin, Germany; Freie Universität Berlin, Berlin, Germany.
Abstract:
Fluorescence resonance energy transfer (FRET) analysis and the recently developed proximity ligation assay (PLA) are widely used to study protein-protein interactions in situ. We have developed correlative FRET-PLA to monitor interactions between membrane proteins that frequently cause problems in confirmatory co-immunoprecipitation assays. Correlative FRET-PLA is particularly aimed at delivering robust and reliable results and is useful for investigating protein-protein interactions.

