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The mouse adenovirus type 1 contains an unusual E3 region
K S Raviprakash1, A Grunhaus, M A el Kholy
1Department of Microbiology-Immunology, Albert Einstein College of Medicine, Bronx, New York 10461.
Abstract:
Since the E3 region of human adenoviruses codes for a series of proteins that are probably involved in viral pathogenesis, the nucleotide sequence for a 3.6-kilobase DNA fragment in the corresponding region (map units 77 through 89) of the mouse adenovirus type 1 genome has been determined. Analysis of the sequence revealed that the genes for the fiber and for the precursor to the hexon-associated protein, pVIII, that usually flank the E3 region, are well conserved. However, many of the open reading frames contained in the E3 region of human adenoviruses between the pVIII and the fiber genes were absent from the mouse adenovirus type 1 genome.
Insights
Mouse adenovirus type 1 lacks many genes found in the human adenovirus E3 region, suggesting differences in viral pathogenesis between species. Conserved flanking genes indicate potential functional importance.
Area of Science:
- Virology
- Genomics
- Comparative Biology
Background:
- The E3 region of human adenoviruses encodes proteins crucial for viral pathogenesis.
- Understanding the genetic makeup of related viruses aids in comparative analysis of disease mechanisms.
Purpose of the Study:
- To determine the nucleotide sequence of the mouse adenovirus type 1 genome's E3 region homolog (map units 77-89).
- To compare the genetic organization of the mouse adenovirus E3 region with that of human adenoviruses.
Main Methods:
- DNA sequencing of a 3.6-kilobase fragment from the mouse adenovirus type 1 genome.
- Bioinformatic analysis of the obtained nucleotide sequence.
Main Results:
- The genes for the fiber protein and the precursor to hexon-associated protein pVIII, which flank the human E3 region, are conserved in mouse adenovirus type 1.
- Numerous open reading frames present in the human adenovirus E3 region were notably absent in the mouse adenovirus type 1 genome.
Conclusions:
- Mouse adenovirus type 1 exhibits significant genomic differences in its E3 region compared to human adenoviruses.
- These genetic variations may contribute to distinct viral pathogenesis mechanisms between these adenovirus types.