Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intravital calcium imaging of meningeal macrophages reveals niche-specific dynamics and aberrant responses to brain hyperexcitability.

eLife·2026

Same author

ACTB methylation regulates SMARCA4 genomic occupancy to promote translation and reduce adhesion in colorectal cancer cells.

Genome research·2026

Same author

E2F1 K117 methylation by SETD6 disrupts BRD4-E2F1 binding and modulates E2F1 chromatin binding and gene regulation in prostate cancer cells.

Nucleic acids research·2026

Same author

cellSTAAR: incorporating single-cell-sequencing-based functional data to boost power in rare variant association testing of noncoding regions.

Nature methods·2025

Same author

Intravital calcium imaging of meningeal macrophages reveals niche-specific dynamics and aberrant responses to brain hyperexcitability.

bioRxiv : the preprint server for biology·2025

Same author

Machine learning-driven prediction of substrates for enzymes introducing or removing protein post-translational modifications.

Communications chemistry·2025

Same journal

Costunolide ameliorates autoimmune uveitis by targeting USP15 to suppress TNF-α-induced retinal endothelial inflammation.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

A ligandable PNT domain establishes ERG as a directly targetable oncogenic driver in prostate cancer.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Identification of cellular intermediates unveils unique enzymes for flagellar glycan biosynthesis in <i>Clostridioides difficile</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

The structure of correlated variability reflects task-relevant information in sensory neurons.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Shared neurogenetic substrates of nonplanning impulsivity and procrastination.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

HIV-1 capsid interactions with Nuclear Pore Complex components support nuclear entry via affinity gradient.

Proceedings of the National Academy of Sciences of the United States of America·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 22, 2026

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

Facilitated sequence counting and assembly by template mutagenesis.

Dan Levy¹, Michael Wigler¹

¹Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724 wigler@cshl.edu levy@cshl.edu.

Proceedings of the National Academy of Sciences of the United States of America

|October 15, 2014

Summary

This summary is machine-generated.

Introducing random mutations to identical DNA templates allows for accurate molecule counting and long-range structure inference, overcoming limitations of short-read sequencing and PCR distortions.

Keywords:

copy number variation expression profiling mutational tagging

More Related Videos

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

Related Experiment Videos

Last Updated: Apr 22, 2026

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Short sequencing reads limit inference of long-range DNA structures.
PCR amplification introduces distortions, affecting accurate template quantification.

Purpose of the Study:

To explore a novel method for distinguishing and counting identical DNA molecules.
To enable accurate long-range DNA structure analysis despite sequence similarity.

Main Methods:

Simulating the introduction of random mutations (cytosine deamination) into identical DNA templates.
Modeling error-free sequencing and perfect mapping to assess mutation utility.
Evaluating the impact of nucleotide conversion and sequence coverage on accuracy.

Main Results:

Accurate counting of otherwise identical DNA molecules is achievable.
Distinguishable patterns allow for connecting variants across long identical sequence spans.
The method is effective under readily achievable conditions.

Conclusions:

This mutation-based approach overcomes key limitations in DNA analysis.
Potential applications include transcript profiling, isoform and genome assembly, and haplotype phasing.