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Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
W D Huse1, L Sastry, S A Iverson
1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Science (New York, N.Y.)
|December 8, 1989
Abstract:
A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.