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Updated: Apr 22, 2026

Production and Testing of Antimicrobial Peptides and Their Mimics
Published on: April 10, 2026
Design of novel analogues of short antimicrobial peptide anoplin with improved antimicrobial activity
Yang Wang1, Jianbo Chen, Xin Zheng
1College of Agronomy, Gansu Agricultural University, Lanzhou, 730000, China.
Abstract:
Currently, novel antibiotics are urgently required to combat the emergence of drug-resistant bacteria. Antimicrobial peptides with membrane-lytic mechanism of action have attracted considerable interest. Anoplin, a natural α-helical amphiphilic antimicrobial peptide, is an ideal research template because of its short sequence. In this study, we designed and synthesized a group of analogues of anoplin. Among these analogues, anoplin-4 composed of D-amino acids displayed the highest antimicrobial activity due to increased charge, hydrophobicity and amphiphilicity. Gratifyingly, anoplin-4 showed low toxicity to host cells, indicating high bacterial selectivity. Furthermore, the mortality rate of mice infected with Escherichia coli was significantly reduced by anoplin-4 treatment relative to anoplin. In conclusion, anoplin-4 is a novel anoplin analogue with high antimicrobial activity and enzymatic stability, which may represent a potent agent for the treatment of infection.
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