Related Experiment Video
Updated: Apr 22, 2026
07:16
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
1.6K
cysTMTRAQ-An integrative method for unbiased thiol-based redox proteomics.
Jennifer Parker1, Kelly Balmant1, Fanchao Zhu2
1From the ‡Department of Biology, Genetics Institute, University of Florida, Gainesville, Florida 32611; §Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32610;
Molecular & Cellular Proteomics : MCP
|October 16, 2014
Summary
We developed cysTMTRAQ, a novel proteomics method for analyzing protein redox changes and total protein levels simultaneously. This approach corrects for protein turnover, ensuring accurate identification of redox-modified cysteines.
Area of Science:
- Proteomics
- Biochemistry
- Molecular Biology
Background:
- Protein redox regulation is crucial for biological processes.
- Cysteine thiols act as redox switches in signaling and metabolic pathways.
- Accurate analysis of post-translational modifications requires accounting for protein turnover.
Purpose of the Study:
- To introduce a novel integrative proteomics method, cysTMTRAQ.
- To enable simultaneous analysis of cysteine redox modifications and total protein levels.
- To accurately identify bona fide redox-modified cysteines by correcting for protein turnover.
Main Methods:
- Integration of cysteine tandem mass tags (TMT) and isobaric tag for relative and absolute quantification (iTRAQ).
- Application of the cysTMTRAQ method in a single experimental workflow.
- Correction for protein turnover to distinguish true modification from changes in protein abundance.
Main Results:
- Simultaneous quantification of cysteine redox state and total protein levels.
- Accurate determination of redox-modified cysteines.
- Validation of the method's ability to provide reliable post-translational modification data.
Conclusions:
- cysTMTRAQ offers a robust approach for studying protein redox regulation.
- Accounting for protein turnover is essential for accurate PTM analysis.
- This method advances proteomic studies in biology and medicine by enabling dynamic analysis of PTMs and protein levels.
