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Related Experiment Videos

Sodium-translocating adenosine triphosphatase in Streptococcus faecalis.

Y Kakinuma1, K Igarashi

  • 1Faculty of Pharmaceutical Sciences, Chiba University, Japan.

Journal of Bioenergetics and Biomembranes
|December 1, 1989
PubMed
Summary

This study reveals that Streptococcus faecalis possesses a sodium-translocating ATPase that is induced by high sodium levels. This enzyme helps bacteria manage sodium in challenging environments.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Cellular Biology

Background:

  • The fermentative bacterium Streptococcus faecalis utilizes ion transport systems for cellular homeostasis.
  • Understanding the role of ion-motive ATPases is crucial for comprehending bacterial adaptation.
  • Previous research has identified various ion-motive ATPases, but the specific sodium-translocating ATPase in S. faecalis requires further elucidation.

Purpose of the Study:

  • To characterize the sodium-translocating ATPase in Streptococcus faecalis.
  • To investigate the regulatory mechanisms and environmental triggers for the induction of this ATPase.
  • To differentiate this enzyme from other known ion-motive ATPases.

Main Methods:

  • Enzyme activity assays measuring sodium-potassium ion exchange.

Related Experiment Videos

  • Investigating enzyme response to varying sodium and potassium ion concentrations.
  • Testing enzyme resistance to specific inhibitors like vanadate and dicyclohexylcarbodiimide.
  • Analyzing enzyme induction under different growth conditions, including high sodium media and limited proton potential generation.

    • Main Results:

    • The sodium-translocating ATPase exchanges sodium for potassium ions, with sodium ions stimulating activity.
    • The enzyme is distinct from E1E2 and F1F0 type ATPases due to its resistance to vanadate and dicyclohexylcarbodiimide.
    • ATPase induction is triggered by high extracellular sodium, especially when proton potential generation is limited or the sodium-proton antiporter is blocked.
    • The enzyme is not induced by potassium deprivation.

    Conclusions:

    • A novel sodium-translocating ATPase in S. faecalis is regulated by intracellular sodium levels.
    • This ATPase plays a role in adapting to sodium-rich environments, particularly when proton motive force is compromised.
    • The enzyme's unique characteristics suggest a distinct evolutionary path for managing ion balance in specific bacterial niches.