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Survivin silencing enhances radiosensitivity in oral squamous cell carcinoma cell

H-B Sun1, H-Y Zheng, X Yan

  • 1Department of Stomatology, the Affiliated Hospital of Qingdao University, Qingdao, Shandong, China. sunhuibinqd@126.com.

European Review for Medical and Pharmacological Sciences
|October 16, 2014
PubMed
Abstract

Insights

Survivin silencing enhances radiosensitivity in oral squamous cell carcinoma (OSCC) cells. Combining survivin knockdown with radiation therapy significantly boosts apoptosis and inhibits tumor growth in vitro and in vivo.

Area of Science:

  • Oncology
  • Molecular Biology
  • Radiotherapy Research

Background:

  • Survivin, an inhibitor of apoptosis protein, is overexpressed in cancers and confers resistance to radiation therapy.
  • Oral squamous cell carcinoma (OSCC) exhibits survivin overexpression, contributing to treatment resistance.

Purpose of the Study:

  • To investigate the effect of survivin knockdown using siRNA on apoptosis induction and radiosensitivity in OSCC cells.
  • To evaluate the combined efficacy of survivin silencing and radiotherapy in vitro and in vivo.

Main Methods:

  • Oral squamous cell carcinoma (OSCC) KB cell lines were treated with radiotherapy and/or survivin-targeting siRNA.
  • Cell viability, proliferation (colony-forming ability), and apoptosis were assessed using MTT assay and flow cytometry.
  • Tumor-bearing mice models were used to evaluate in vivo efficacy.

Main Results:

  • Survivin knockdown reduced cell proliferation and increased radiation-induced apoptosis in KB cells.
  • Apoptosis was augmented by survivin silencing, especially when combined with irradiation.
  • Colony formation was significantly inhibited by the combination of survivin silencing and irradiation.

Conclusions:

  • Survivin silencing sensitizes OSCC cells to radiotherapy, enhancing apoptosis and inhibiting proliferation and colony formation.
  • The combination of survivin silencing and radiation therapy demonstrates significant therapeutic potential in both in vitro and in vivo models of OSCC.
  • Survivin silencing potentiates the anti-tumor effects of irradiation, offering a promising strategy for improving OSCC treatment outcomes.

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