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Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
The complete mitochondrial genome sequence of Hemibagrus sp. (Siluriformes: Bagridae), molecular data for species
Can-Lin Zhou1,2, Juan Tian3, Chang-Geng Yang4
1a College of Life Science and Technology, Xinjiang University , Urumqi , P.R. China .
Abstract:
In this study, we determined and described the complete mitogenome sequence of Hemibagrus sp. for the first time, which is 16,513 bp in length, and contains 37 genes, including 13 protein-coding genes, 2 rRNAs, 22 tRNAs, 1 origin of replication on the light-strand (OL) and a putative control region. The overall base composition was 31.1% A, 26.9% T, 26.9% C, 15.1% G, with a slight AT bias (58.0%). All protein-coding genes shared the start codon ATG, except for COI, which began with GTG. The tRNA-Ser(UGC) couldn't be folded into the typical cloverleaf secondary structure because its dihydrouridine arm is replaced by a simple loop. These results are expected to provide useful molecular data for species identification and further phylogenetic studies of Bagridae and Siluriformes.
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