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Methodology for Accurate Detection of Mitochondrial DNA Methylation
Published on: May 20, 2018
Analysis of the Rongshui Xiang duck (Anseriformes, Anatidae, Anas) mitochondrial DNA
Yanfang Zhang1, Zhixun Xie1, Liji Xie1
1a Department of Biotechnology, Guangxi Key Laboratory of Animal Vaccines and Diagnostics , Guangxi Veterinary Research Institute , Nanning , Guangxi , P.R. China.
Abstract:
The complete mitochondrial genome sequence of the Rongshui Xiang duck was measured by PCR-based methods. Our research findings reveal that the entire mitochondrial genome of the Rongshui Xiang duck is a circular molecule consisting of 16,605 bp (GenBank accession number: KJ833587). The contents of A, T, C, and G in the mitochondrial genome were found to be 29.20%, 22.20%, 32.82% and 15.79%, respectively, similar to the majority of avian species. The complete mitochondrial genome of the Rongshui Xiang duck contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 control region. The characteristics of the mitochondrial genome were analyzed in detail. Our complete mitochondrial genome sequence should provide essential information for understanding phylogenetic relationships among duck mitochondrial genomes.
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