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Updated: Apr 22, 2026
Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
Published on: September 19, 2018
Analysis of changes in phosphorylation of receptor tyrosine kinases: antibody arrays
Sweta Rani1, Lorraine O'Driscoll
1School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, East End Development 4/5, Trinity College Dublin, Dublin-2, Ireland, sweta.rani@nuigalway.ie.
Abstract:
Tyrosine kinases are mainly classified into two groups, as receptor tyrosine kinase (RTK) and non-receptor tyrosine kinase (NRTK). The RTK family of transmembrane ligand-binding proteins are important mediators of the signaling cascade and includes EGFR, PDGFR (platelet-derived growth factor receptors), FGFR (fibroblast growth factor receptor) and the IR (insulin receptor). RTKs comprise 59 members and their structure includes an extracellular ligand-binding domain, a transmembrane domain, and an intracellular domain possessing the tyrosine kinase activity. This chapter focuses on antibody arrays that are basically used to analyse phosphorylation and dephosphorylation of RTKs. Antibody arrays include well-characterized antibodies for profiling, changes in RTK expression, and comparison between normal, diseased, or treated samples.
Insights
Receptor tyrosine kinases (RTKs) are key in cell signaling. Antibody arrays offer a method to study RTK phosphorylation and expression changes in various conditions.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Signaling
Background:
- Tyrosine kinases are crucial enzymes, classified as receptor tyrosine kinases (RTKs) and non-receptor tyrosine kinases (NRTKs).
- The RTK family, comprising 59 members, plays a vital role in cellular signaling cascades, with examples including EGFR, PDGFR, FGFR, and IR.
- RTKs possess a characteristic structure with extracellular ligand-binding, transmembrane, and intracellular tyrosine kinase domains.
Purpose of the Study:
- To explore the application of antibody arrays in analyzing receptor tyrosine kinase (RTK) activity.
- To highlight the utility of antibody arrays for profiling RTK expression and phosphorylation states.
Main Methods:
- Utilizing antibody arrays for the analysis of receptor tyrosine kinase (RTK) phosphorylation and dephosphorylation.
- Employing well-characterized antibodies within antibody arrays for comprehensive RTK profiling.
Main Results:
- Antibody arrays enable the detailed analysis of RTK phosphorylation status.
- These arrays facilitate the profiling of RTK expression levels.
Conclusions:
- Antibody arrays are effective tools for investigating RTK signaling pathways.
- This methodology allows for the comparison of RTK profiles across normal, diseased, and treated biological samples.
