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Polymorphonuclear cell phagocytosis and surface receptor modulation after extracorporeal circulation.
G Busnach1, B Brando, A Cappelleri
1Department Nephrology, Niguarda Ca'Granda Hospital, Milan, Italy.
ASAIO Transactions
|July 1, 1989
Summary
Extracorporeal circulation (EC) during hemodialysis (HD) or plasma exchange (PE) temporarily increases C3bi receptor density on polymorphonuclear cells (PMNs). This procedure also depresses PMN phagocytic activity, suggesting a link to membrane biocompatibility.
Area of Science:
- Immunology
- Nephrology
Background:
- Polymorphonuclear cells (PMNs) play a crucial role in immune response.
- Hemodialysis (HD) and plasma exchange (PE) involve extracorporeal circulation (EC), potentially affecting immune cell function.
Purpose of the Study:
- To investigate the impact of EC on PMN phagocytic capacity and Fc-gamma and C3bi receptor expression.
- To determine if observed changes are related to the specific EC procedure or other factors.
Main Methods:
- Flow cytometry was used to analyze PMN receptor expression (Fc-gamma and C3bi) and phagocytic capacity.
- Fluorescent microbeads and specific monoclonal antibodies (Leu11c, Leu15) were employed.
- Analysis was performed before and after HD and PE procedures.
Main Results:
- Fc-gamma receptor expression decreased overall in HD and PE patients but was unaffected by EC itself.
- C3bi receptor density on PMNs increased post-EC, independent of HD/PE technique or disease.
- Phagocytosis by PMNs was qualitatively and quantitatively depressed after EC.
Conclusions:
- EC induces a temporary increase in PMN C3bi receptor density and a decrease in phagocytic activity.
- These changes appear to be procedure-related and may be linked to membrane biocompatibility.
- Further research is needed to fully elucidate the mechanisms and clinical implications.