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Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein
Published on: April 21, 2015
Swine interferon-inducible transmembrane proteins potently inhibit influenza A virus replication
Caroline Lanz1, Emilio Yángüez2, Dario Andenmatten2
1Institute of Medical Virology, University of Zurich, Zurich, Switzerland Life Sciences Zurich Graduate School, ETH and University of Zurich, Zurich, Switzerland.
Abstract:
Human interferon-inducible transmembrane proteins (IFITMs) were identified as restriction factors of influenza A virus (IAV). Given the important role of pigs in the zoonotic cycle of IAV, we cloned swine IFITMs (swIFITMs) and found two IFITM1-like proteins, one homologue of IFITM2, and a homologue of IFITM3. We show that swIFITM2 and swIFITM3 localize to endosomes and display potent antiviral activities. Knockdown of swIFITMs strongly reduced virus inhibition by interferon, establishing the swIFITMs as potent restriction factors in porcine cells.
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