Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered sarcoplasmic reticulum function after high-intensity exercise.

S K Byrd1, L J McCutcheon, D R Hodgson

  • 1Department of Veterinary and Comparative Anatomy, College of Veterinary Medicine, Washington State University, Pullman 99164-6520.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|November 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracorporeal shockwave therapy raises mechanical nociceptive threshold in horses with thoracolumbar pain.

Equine veterinary journal·2019
Same author

Pharmacokinetics and safety of repeated oral dosing of acetaminophen in adult horses.

Equine veterinary journal·2019
Same author

PD-L1 and immune infiltrates are differentially expressed in distinct subgroups of gastric cancer.

Oncoimmunology·2019
Same author

Effects of age and diet on glucose and insulin dynamics in the horse.

Equine veterinary journal·2018
Same author

Pharmacokinetics of firocoxib after intravenous administration of multiple consecutive doses in neonatal foals.

Journal of veterinary pharmacology and therapeutics·2017
Same author

Lamellar events related to insulin-like growth factor-1 receptor signalling in two models relevant to endocrinopathic laminitis.

Equine veterinary journal·2017
Same journal

Thoroughbred horses susceptible to Recurrent Exertional Rhabdomyolysis have elevated skeletal muscle mitochondrial capacities.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Change in Neutrophil-to-Lymphocyte Ratio after acute and chronic exercise: A Systematic Review and Meta-Analysis.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Ankylosing spondylitis and muscle sympathetic nerve activity: a case study.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Intracranial vasomotor and blood flow responses to light intensity aerobic exercise in young adults: a 4D flow MRI study.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Comparative assessments of the COSMED adaptive mixing chamber vs. breath-by-breath methods for oxygen uptake measurements in recreationally active adults.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Can we assess exercise metabolism from skin? Metabolomic profiles in skin dialysate collected during exercise.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
See all related articles

High-intensity exercise temporarily impairs sarcoplasmic reticulum function in horses, affecting calcium uptake and ATPase activity. However, these crucial muscle functions fully recover within 60 minutes post-exercise.

Area of Science:

  • Exercise Physiology
  • Muscle Biology
  • Biochemistry

Background:

  • Sarcoplasmic reticulum (SR) plays a vital role in muscle contraction by regulating calcium (Ca2+) levels.
  • High-intensity exercise can induce significant physiological stress on skeletal muscle.
  • Understanding the impact of exercise on SR function is crucial for athletic performance and recovery.

Purpose of the Study:

  • To investigate the effects of acute high-intensity exercise on the rate and capacity of Ca2+ uptake by the SR.
  • To assess the Ca2+-stimulated adenosinetriphosphatase (ATPase) activity of the SR post-exercise.
  • To determine the reversibility of exercise-induced changes in SR function.

Main Methods:

  • Thoroughbred horses underwent maximal O2 uptake exercise on a treadmill until fatigue.

Related Experiment Videos

  • Muscle biopsies and blood samples were collected at rest, immediately post-exercise, and 30 and 60 minutes post-exercise.
  • Assessed Ca2+ uptake, Ca2+-stimulated ATPase activity, muscle temperature, and pH.

    • Main Results:

    • Muscle lactate concentration and temperature significantly increased immediately post-exercise.
    • Both the rate and capacity of Ca2+ uptake and Ca2+-stimulated ATPase activity were significantly depressed immediately after exercise.
    • SR function (Ca2+ uptake and ATPase activity) returned to baseline levels by 60 minutes post-exercise.

    Conclusions:

    • Acute high-intensity exercise transiently impairs sarcoplasmic reticulum Ca2+ handling and associated ATPase activity in equine skeletal muscle.
    • These impairments are likely induced by acute changes in local muscle pH and temperature.
    • Sarcoplasmic reticulum function demonstrates a robust capacity for recovery following intense exercise.