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[The interaction between ononin and human intestinal bacteria].

Wei Zhang, Shu Jiang, Da-Wei Qian

    Yao Xue Xue Bao = Acta Pharmaceutica Sinica
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    Human intestinal bacteria metabolize ononin through various pathways, influencing gut flora. Ononin inhibits pathogens while promoting beneficial probiotics, impacting the intestinal microbial balance.

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    Area of Science:

    • Microbiology
    • Pharmacology
    • Metabolomics

    Background:

    • Ononin, a plant isoflavone, has potential health benefits.
    • Understanding its metabolism by gut bacteria is crucial for its efficacy.
    • Gut microbiota plays a significant role in host health and xenobiotic metabolism.

    Purpose of the Study:

    • To investigate the metabolic pathways of ononin by human intestinal bacteria.
    • To assess the impact of ononin on the growth of key intestinal bacterial species.
    • To determine how ononin affects the balance of gut microbiota.

    Main Methods:

    • Culturing of 69 distinct bacterial colonies from human fecal samples.
    • Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) for metabolite analysis.
    • Microplate reader assays to quantify bacterial growth (Enterococcus, Enterobacter, Lactobacilli, Bifidobacteria).

    Main Results:

    • Identified major metabolic pathways: hydrogenation, demethylation, hydroxylation, and deglycosylation.
    • Ononin demonstrated inhibitory effects on pathogenic bacteria (Enterococcus, Enterobacter).
    • Ononin promoted the growth of probiotic bacteria (Bifidobacteria, Lactobacilli).

    Conclusions:

    • Human intestinal bacteria possess the capability to metabolize ononin via biotransformation.
    • Ononin influences the intestinal flora balance by differentially affecting pathogen and probiotic growth.
    • The extent of ononin's effect on gut microbiota varies with bacterial species and concentration.