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Phenotypic modulation of mesenteric vascular smooth muscle cells from type 2 diabetic rats is associated with

Maria Alicia Carrillo-Sepulveda1, Takayuki Matsumoto

  • 1Department of Physiology, Georgia Regents University, Augusta, GA, USA.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|October 18, 2014
PubMed
Abstract

Insights

Diabetic mesenteric vascular smooth muscle cells (VSMCs) show reduced caveolin-1, promoting a proliferative phenotype. This vascular smooth muscle cell modulation is linked to increased inflammation and glucose levels in diabetes.

Area of Science:

  • Vascular Biology
  • Cellular Metabolism
  • Diabetes Complications

Background:

  • Vascular smooth muscle cell (VSMC) phenotypic modulation contributes to diabetes-associated vascular complications.
  • Loss of caveolin-1 expression is implicated in VSMC proliferation.

Purpose of the Study:

  • To investigate if mesenteric VSMCs from type 2 diabetic Goto-Kakizaki (GK) rats exhibit phenotypic modulation linked to decreased caveolin-1 expression.
  • To explore the role of inflammatory stimuli and glucose in these diabetic vascular changes.

Main Methods:

  • Isolated mesenteric VSMCs from GK and Wistar rats.
  • Western blotting for caveolin-1, calponin, and PCNA.
  • Assessed reactive oxygen species (ROS) and ERK1/2 activation.

Main Results:

  • Diabetic GK rat VSMCs showed reduced caveolin-1 and calponin, increased PCNA, elevated ROS, and phospho-ERK1/2.
  • LPS and high glucose exacerbated these changes in VSMCs.

Conclusions:

  • Mesenteric VSMCs in diabetic GK rats undergo phenotypic modulation associated with decreased caveolin-1.
  • Enhanced inflammation and glucose levels likely drive these diabetic vascular alterations.

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